The Surveillance and Information Sharing Operational Tool (SIS OT) was developed by the Tripartite organizations (FAO, WHO and OIE) and technical experts to support national authorities to establish or strengthen a One Health multisectoral coordinated surveillance and information sharing system for zoonotic diseases. It provides a stepwise assessment to evaluate a country's existing surveillance capacity, identify activities and create a roadmap and workplan, while linking users to a toolbox of existing resources. Staff from national ministries responsible for management of zoonotic diseases will find it useful to increase the level of coordinated surveillance capacity.